Microfluidic: An innovative tool for efficient cell sorting

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.

We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS) combining many of the advantages of microtitre-plate screening and traditional fluorescence-activated cell sorting (FACS). Single cells are compartmentalized in emulsion droplets, which can be sorted using dielectrophoresis in a fluorescence-activated manner (as in FACS) at rates up to 2000 droplets s(-1). ...

Fluorescence-Activated Cell Sorting in Microfluidic Devices

Moss – An Innovative Tool for Protein Production

The complete sequencing of the human genome has significantly increased the number of pharmaceutically important proteins (“biopharmaceuticals”) to be produced at large scale. As this tendency is expected to continue, production capacity of biopharmaceuticals has become a strategic issue for the pharmaceutical industry. Mammalian cells such as those of baby hamster kidney and Chinese hamster ov...

PROMPT: An Innovative Design Tool

We describe a system, Prompt, used to design physical systems. Prompt employs a multi-level approach to design. When simple constraint propagation over prototypes [Adda fails, Prompt can significantly modify prototypes by reasoning about their structure and physics. Prompt derives the behavior of a prototype from its structure using knowledge of physics stored in a Graph of Models. It then uses...

An efficient external sorting algorithm

This paper presents an optimal external sorting algorithm for two-level memory model. Our method is different from the traditional external merge sort and it uses the sampling information to reduce the disk I/Os in the external phase. The algorithm is efficient, simple and it makes a good use of memory available in the recent computer environment. Under the certain memory constraint, this algor...